Documentation Overview
Peptide Hackers provides analytical documentation to support research quality, traceability, and reproducibility. Each product batch is accompanied by laboratory-generated data produced using validated analytical methods appropriate for research-grade materials.
All documentation is batch-specific and applies only to the referenced production lot.
Our Three Pillars of Peptide Quality
Peptide Hackers evaluates peptide quality across three critical dimensions: purity, quantity accuracy, and laboratory standards. Each batch is assessed against these criteria prior to release for research use.
≥99% by HPLC
Peptides are manufactured to high research-grade purity standards. Most products meet or exceed 99% purity as determined by reversed-phase HPLC analysis. Purity results are documented on each batch-specific Certificate of Analysis and reflect only the tested lot.
Content verified per vial
When a vial is labeled as 10 mg, the peptide content is measured to be very close to the stated amount. Significant deviation — receiving 7 mg or 15 mg when labeled 10 mg — is unacceptable. Peptide content is verified using validated analytical methods, and minimum peptide content specifications are defined on the Certificate of Analysis.
Independent third-party testing
Certificates come from independent labs: Accumark Labs (Anaheim, CA) reports HPLC purity, identity and quantity per vial; Janoshik Analytical (Prague) reports HPLC purity and quantity. Accumark lists its ISO/IEC 17025 accreditation as pending, and Janoshik makes no accreditation claim. Each certificate links to the lab's own record.
ISO/IEC 17025 accreditation, where held, confirms technical competence, validated methods, calibrated instrumentation, and documented quality controls. We publish each lab's accreditation status exactly as the labs state it: Accumark lists its accreditation as pending, and Janoshik makes no accreditation claim on its reports or its site.
Certificate of Analysis
Each production lot is issued a Certificate of Analysis summarizing analytical test results for that specific batch. COAs include, where applicable:
- Product name and batch or lot number
- Date of analysis and testing laboratory reference
- Purity determination by HPLC
- Identity result — the lab's confirmation that the sample is the named compound (Accumark reports)
- Additional quality attributes as applicable
- Specification limits alongside measured results
COAs reflect results obtained from the tested batch only and are not transferable to other lots. For help reading one, see understanding Certificates of Analysis.
Analytical Methods
HPLC
Purity is determined using reversed-phase HPLC with UV detection, typically monitored at 214–220 nm, on C18 columns with standard gradient methods. Quantitative purity is calculated by peak area integration and reported as a percentage of total detected peak area.
Confirms: purityIdentity
Where a lab reports identity, it is that lab's confirmation that the sample is the named compound. Our Accumark AccuVerify certificates include this identity result; Janoshik certificates cover purity and quantity. The certificates do not state which instrument or method the lab used.
Reported: identity (Accumark COAs)Amino Acid Analysis
Where specified, amino acid composition is verified through acid hydrolysis followed by chromatographic quantitation, confirming the presence and expected ratios of constituent amino acids.
Confirms: compositionKarl Fischer Titration
Moisture content is determined where applicable and reported as a percentage of total mass; it may be considered in peptide content calculations.
Confirms: water contentLAL Endotoxin Test
Where performed, bacterial endotoxin levels are assessed using the Limulus amebocyte lysate assay and reported as endotoxin units per milligram.
Confirms: endotoxin levelSpecifications
Product specifications represent acceptance criteria established based on analytical method performance and research-grade quality standards. Not all tests apply to every product; applicable specifications are listed on the corresponding Certificate of Analysis.
| Parameter | Typical specification | Method |
|---|---|---|
| Purity | ≥99% (minimum HPLC purity) | Reversed-phase HPLC |
| Identity | The lab's confirmation of the named compound | Accumark AccuVerify reports |
| Peptide content | ≥80% active peptide | Validated quantitative method |
| Water content | ≤8% moisture | Karl Fischer titration |
| Endotoxin | Maximum level where tested | LAL assay |
Documentation Access
Certificates of Analysis are available through the COA section of this website. Each document is identified by a unique reference tied to the product batch number.
Frequently Asked Questions
What purity are Peptide Hackers peptides?
Most products meet or exceed 99% purity as determined by reversed-phase HPLC analysis. The exact purity for the batch you received is printed on its Certificate of Analysis, which reflects only the tested lot.
How is peptide identity confirmed?
Accumark's AccuVerify reports include an identity result — the lab's confirmation that the sample is the named compound. The certificates don't state a mass spectrometry method. Janoshik reports cover purity and quantity only.
What does peptide content mean on a COA?
Peptide content is the mass fraction of actual peptide in the lyophilized vial; the remainder is counterions, water, and residual solvents. Peptide Hackers specifies a minimum content (typically at least 80%) so the labeled amount closely matches what is in the vial.
Who performs the analytical testing?
Testing is performed by independent third-party labs: Accumark Labs (Anaheim, CA) reports HPLC purity, identity and quantity per vial and lists its ISO/IEC 17025 accreditation as pending; Janoshik Analytical (Prague) reports HPLC purity and quantity. Every certificate links to the lab's own record.
How do I find the COA for my batch?
Every batch has a Certificate of Analysis identified by its batch or lot number. COAs can be browsed in the COA section of this website and referenced by batch number for traceability.
Can this documentation be used for regulatory submission?
No. Documentation supports research activities only — identity verification, traceability, methodology records, and audit support. It does not constitute certification, approval, or authorization for pharmaceutical, clinical, diagnostic, or human-use applications.
Documentation Purpose and Limitations
Technical documentation is provided solely to support research activities, including:
- Verification of material identity and quality
- Laboratory record-keeping and traceability
- Research methodology documentation
- Institutional compliance and audit support
Documentation is not intended for regulatory submission and does not constitute certification, approval, or authorization for pharmaceutical, clinical, diagnostic, or human-use applications. See our research use statement and disclaimer.