Documentation Overview
Peptide Hackers provides analytical documentation to support research quality, traceability, and reproducibility. Each product batch is accompanied by laboratory-generated data produced using validated analytical methods appropriate for research-grade materials.
All documentation is batch-specific and applies only to the referenced production lot.
Our Three Pillars of Peptide Quality
Peptide Hackers evaluates peptide quality across three critical dimensions: purity, quantity accuracy, and laboratory standards. Each batch is assessed against these criteria prior to release for research use.
Purity
Peptides are manufactured to high research-grade purity standards. Most products meet or exceed 99% purity as determined by reversed-phase HPLC analysis. Purity results are documented on each batch-specific Certificate of Analysis and reflect only the tested lot.
Quantity Accuracy
Peptide content accuracy matters. When a vial is labeled as 10 mg, the peptide content is measured to be very close to the stated amount. Significant deviation, such as receiving 7 mg or 15 mg when labeled as 10 mg, is unacceptable.
Peptide content is verified using validated analytical methods, and minimum peptide content specifications are defined on the Certificate of Analysis. This ensures researchers receive material that closely matches the labeled quantity for reproducible experimental work.
Laboratory Standards
All analytical testing is performed by an independent laboratory accredited to ISO/IEC 17025, the highest international standard for testing and calibration laboratories. Manufacturing partners operate under ISO 9001:2015 quality management systems where applicable.
ISO/IEC 17025 accreditation confirms technical competence, validated methods, calibrated instrumentation, and documented quality controls. ISO 9001:2015 certification ensures consistent manufacturing processes, traceability, and quality management oversight.
Together, these standards support consistent purity, accurate labeling, and reliable analytical documentation across product batches.
Certificate of Analysis
Each production lot is issued a Certificate of Analysis summarizing analytical test results for that specific batch. COAs include, where applicable:
- Product name and batch or lot number
- Date of analysis and testing laboratory reference
- Purity determination by HPLC
- Molecular identity confirmation by mass spectrometry
- Additional quality attributes as applicable
- Specification limits alongside measured results
COAs reflect results obtained from the tested batch only and are not transferable to other lots.
Analytical Methods
High-Performance Liquid Chromatography (HPLC)
Purity is determined using reversed-phase HPLC with UV detection, typically monitored at 214–220 nm. Quantitative purity is calculated by peak area integration and reported as a percentage of total detected peak area. Standard gradient methods utilizing C18 columns are employed.
Mass Spectrometry
Molecular identity is confirmed using mass spectrometry techniques such as ESI-MS or MALDI-TOF. Observed molecular mass is compared against theoretical mass calculated from the peptide sequence. Agreement within accepted analytical tolerances confirms identity.
Amino Acid Analysis
Where specified, amino acid composition is verified through acid hydrolysis followed by chromatographic quantitation. Results confirm the presence and expected ratios of constituent amino acids.
Water Content
Moisture content is determined by Karl Fischer titration where applicable. Results are reported as a percentage of total mass and may be considered in peptide content calculations.
Endotoxin Testing
Where performed, bacterial endotoxin levels are assessed using Limulus amebocyte lysate assay. Results are reported as endotoxin units per milligram.
Specifications
Product specifications represent acceptance criteria established based on analytical method performance and research-grade quality standards. Specifications may include:
- Purity: Minimum HPLC purity, typically ≥99%
- Identity: Molecular mass within defined tolerance
- Peptide Content: Minimum percentage of active peptide, typically ≥80%
- Water Content: Maximum moisture content, typically ≤8%
- Endotoxin: Maximum endotoxin level where tested
Not all tests apply to every product. Applicable specifications are listed on the corresponding Certificate of Analysis.
Documentation Access
Certificates of Analysis are available through the COA section of this website. Each document is identified by a unique reference tied to the product batch number.
Documentation Purpose and Limitations
Technical documentation is provided solely to support research activities, including:
- Verification of material identity and quality
- Laboratory record-keeping and traceability
- Research methodology documentation
- Institutional compliance and audit support
Documentation is not intended for regulatory submission and does not constitute certification, approval, or authorization for pharmaceutical, clinical, diagnostic, or human-use applications.