PE-22-28
PH-PE2228-001
Specifications
Description
PE-22-28 is a heptapeptide derived from the spadin sequence, identified as a potent TREK-1 potassium channel blocker with antidepressant properties.
Mechanism of Action
PE-22-28 inhibits TREK-1 (TWIK-related potassium channel 1), a two-pore domain potassium channel involved in neuronal excitability and mood regulation. By blocking TREK-1, this peptide enhances neuronal firing and mediates antidepressant-like effects in preclinical models. PE-22-28 displays better specificity and affinity for TREK-1 channel compared to spadin, with significantly improved in vivo stability and antidepressant activity (Djillani et al., 2017). Spadin, from which PE-22-28 is derived, was identified as the first natural antidepressant peptide that specifically blocks TREK-1 channels (Mazella et al., 2010).
Research Applications
- TREK-1 channel pharmacology and drug discovery (Djillani et al., 2017)
- Novel antidepressant mechanism studies (Mazella et al., 2010)
- Two-pore domain potassium channel research
- Mood disorder and anxiety models
- Neuropsychiatric target validation
Product Specifications
- Purity: ≥99% (HPLC verified)
- Molecular Weight: ~800 Da
- Form: Lyophilized powder
- Storage: -20°C
References
- Djillani A, et al. "Shortened Spadin Analogs Display Better TREK-1 Inhibition, In Vivo Stability and Antidepressant Activity." Front Pharmacol. 2017. PMID: 28955242
- Mazella J, et al. "Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: a new concept in the antidepressant drug design." PLoS Biol. 2010. PMID: 20405001
Documentation
Storage and Handling
- ❄️ Store lyophilized peptide at -20°C or below
- 🔒 Protect from light and moisture
- 🌡️ Allow vial to reach room temperature before opening
- 💧 Reconstitute with appropriate sterile solvent
- 📦 Aliquot to avoid repeated freeze-thaw cycles
Research Overview
PE-22-28 is a synthetic peptide compound used in laboratory research settings. Researchers studying cognitive function and neuroprotection use this compound to investigate cellular signaling pathways, receptor binding affinities, and structure-activity relationships. Each batch is manufactured under controlled conditions and verified through HPLC and mass spectrometry analysis to ensure consistency across experimental replicates.
Documentation & Resources
- Certificate of Analysis for PE-22-28 — batch-specific purity and identity verification
- Research Blog — protocols, guides, and peptide research literature
- Research Use Policy — handling and compliance guidelines
