B12
PH-B12
Specifications
- Purity (HPLC)
- ≥98%
- Size
- 10ml
- Molecular Weight
- 1344.4 g/mol (Methylcobalamin)
- Catalog No.
- PH-B12
Description
B12 (methylcobalamin) is a research-grade form of vitamin B12 for studies of cellular energy metabolism, methylation pathways, and neurological function. Methylcobalamin is the bioactive coenzyme form of B12 and is used directly by cells without hepatic conversion.
Mechanism of Action
Vitamin B12 acts as a cofactor for two key enzymes: methionine synthase, which regenerates methionine from homocysteine and supplies methyl groups for DNA, protein, and lipid methylation; and methylmalonyl-CoA mutase, which supports mitochondrial energy production. Both intramuscular and sublingual routes achieve effective repletion in deficiency models (Sharabi et al., 2019; Parry-Strong et al., 2016).
Research Applications
- Energy metabolism and fatigue studies
- Methylation and homocysteine research
- Neurological and cognitive function
- Route-of-administration comparison (intramuscular vs. sublingual)
References
- Sharabi A, et al. "Replacement therapy for vitamin B12 deficiency: comparison between the sublingual and oral route." Br J Clin Pharmacol. 2019. PMID: 30632091
- Parry-Strong A, et al. "Sublingual vitamin B12 compared to intramuscular injection in patients with type 2 diabetes treated with metformin." N Z Med J. 2016. PMID: 27355231
Verification
Storage & Handling
Storage
B12 is supplied as a lyophilized powder. Keep it sealed, dry, and away from light until reconstitution.
Reconstitution
Let the vial reach room temperature before opening, then add solvent slowly down the vial wall — do not shake.
Stability
Lyophilized material is stable when frozen. Once reconstituted, keep it cold and minimise handling to preserve integrity.
Research Overview
B12 is a synthetic peptide compound used in laboratory research settings. Researchers studying metabolic regulation and energy homeostasis use this compound to investigate cellular signaling pathways, receptor binding affinities, and structure-activity relationships. Each batch is manufactured under controlled conditions and verified through HPLC and mass spectrometry analysis to ensure consistency across experimental replicates.
Documentation & Resources
- Certificate of Analysis for B12 — batch-specific purity and identity verification
- Research Blog — protocols, guides, and peptide research literature
- Research Use Policy — handling and compliance guidelines
Frequently Asked Questions
What purity is B12?
Each lot of B12 is verified to ≥98% by HPLC and confirmed by mass spectrometry. Results are documented in a batch-specific Certificate of Analysis.
Does B12 include a Certificate of Analysis?
Yes. A batch-specific Certificate of Analysis for B12 documents its purity and identity and is available on our COA page.
How should B12 be stored?
B12 is supplied as a lyophilized powder. For short-term storage keep it refrigerated; for long-term storage keep it frozen, away from light and moisture.
How is B12 shipped?
Orders ship at a $15 flat rate, free on orders over $199, in discreet, insulated packaging. Domestic delivery typically takes 1–3 business days.
Can B12 be used on humans or animals?
No. B12 is supplied strictly for in-vitro laboratory research. It is not for human or veterinary use, consumption, or diagnostic or clinical applications.
